Esposito takes over from Mary Anne Hersam, who served in 2012-13.
Tucker Murphy, executive director of the chamber, said, "I am confident that Jenny will be a terrific president, and with three new board members, we have a lot of new energy and ideas to build upon."
The new board members are Brian Lewis, Lisa Corrado and Steve Bazewicz.
Lewis is the owner and executive chef of elm restaurant, Corrado owns Lisa Corrado Nutrition and Steve Bazewicz is the owner of 360 Fitness.
The three new members work in categories of business that were not previously represented on the chamber board.